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Cas12a（又称cpf1）是一类由单个crrna介导的核酸内切酶，能特异性识别并剪切带pam（protospacer adjacent motif，即原 … Cas12a对靶标双链dna的剪切位点主要取决于pam，原有研究显示，cas12a在非靶标链 (nts)距pam第18位和靶标链 (ts)距pam …